Antibacterial, Antioxidant, and Antiproliferative Activities of Corymbia citriodora and the Essential Oils of Eight Eucalyptus Species

[Background]: Essential oils (EOs) have shown antimicrobial, antioxidant, and antiproliferative activity, which may, alone or in combination with other substances, potentially be used for the development of new drugs. However, their chemical variability, depending on the species, varieties, or geographical origin (among other factors) determines different bioactivities that need to be evaluated.[Methods]: The antioxidant activity of Corymbia citriodora and eight Eucalyptus species EOs was determined using two different methods: the scavenging ability of 2,20-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+•) and peroxyl free radicals or oxygen radical absorbance capacity (ORAC). Antibacterial activity was evaluated using the microorganisms Streptococcus pneumoniae (strains D39 and TIGR4), and Haemophilus influenza (strain DSM 9999). The essential oils’ minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was assessed using a microdilution method. The antiproliferative activity was determined using the THP-1 cell line (human acute monocytic leukaemia) with methylthiazolyldiphenyl-tetrazolium bromide assay (MTT).[Results]: Corymbia citriodora and Eucalyptus viminalis EOs showed the highest ABTS and peroxyl free radical scavenging capacity. Eucalyptus globulus EO showed a high potential to treat Streptococcus pneumoniae infections. Haemophilus influenzae was the respiratory pathogen that showed the highest resistance to all EOs, including tea tree EO. After 96 h of incubation, at 25 µg/mL, Eucalyptus radiata and Eucalyptus viminalis EOs showed highest cytotoxic activity against the THP-1 cell line.[Conclusions]: Despite their specific bioactivities, no single EO showed simultaneously good antioxidant, antimicrobial, and antiproliferative activity.This study was partially funded by CESAM (UID/AMB/50017-POCI-01-0145-FEDER-007638), to FCT/MCTES through national funds (PIDDAC), and co-funding by the FEDER within the PT2020 Partnership Agreement and Compete 2020, and by the Fundação para a Ciência e a Tecnologia (FCT), under the projects UID/BIA/04325/2013—MEDTBIO, and UID/BIM/04773/2013 CBMRPeer reviewe

Actuación de la masoterapia en pacientes oncológicos con dolor: estudio de caso

El presente estudio tiene como objetivo verificar la acción de la terapia de masaje en un paciente oncológico con dolor incontrolado en términos de signos vitales y efectos generales reportados después de las sesiones. Un paciente accedió al grupo de terapia de masaje del Coimbra IPOFG en el período comprendido entre febrero y marzo de 2011. Este paciente fue sometido a un estudio de caso estudio de caso que evaluó los signos vitales, en tres sesiones espaciadas una semana, en dos momentos antes y después de la aplicación de la terapia de masaje. La evaluación ha permitido realizar una comparación transversal, que analiza los resultados entre sesiones y una comparación longitudinal que permitió evaluar los resultados entre los dos momentos de evaluación realizada en las tres sesiones. Se concluyó que había diferencias más evidentes entre la primera sesión y la tercera. Se obtuvieron niveles libres de dolor en la última sesión. El participante del estudio informa, al final de las tres sesiones, mejoras generalizadas, habiendo podido prescindir del antidepresivo que ingería regularmente.The present study aims to verify the action of massage therapy in an oncological oncological patient with uncontrolled pain in terms of vital signs and general effects reported after sessions. A patient who accessed the massage therapy group of the Coimbra IPOFG in the period between February and March 2011. This patient was subjected to a case study case study that assessed her vital signs, in three sessions spaced one week apart, at two moments moments: before and after the application of massage therapy. The evaluation made it possible to carry out a transversal comparison, which analyses the results between sessions and a longitudinal comparison which made it possible to evaluate the results between the two moments of evaluation performed in the three sessions. It was concluded that there were more evident differences between the first session and the third, the pain-free levels were obtained in the last session. The participant of the study reports, at the end of the three sessions, generalised improvements, having been able to do without the antidepressant that regularly ingested.O presente estudo tem como objectivo verificar a acção da massagem terapêutica num doente oncológico com dor não controlada ao nível dos sinais vitais e efeitos gerais relatados após sessões. Foi seleccionada uma utente que acedeu pela primeira vez ao grupo de massagem terapêutica do IPOFG Coimbra no período temporal de Fevereiro e Março de 2011. Esta foi sujeita a um estudo de caso que avaliava os sinais vitais, em três sessões espaçadas, com uma semana entre si, em dois momentos: antes da aplicação da massagem terapêutica e depois da aplicação desta. A avaliação permitiu realizar uma comparação transversal, que analisa os resultados entre sessões e uma comparação longitudinal que permitiu avaliar os resultados entre os dois momentos de avaliação realizados nas três sessões. Concluiu-se que existiram diferenças mais evidentes entre a primeira sessão e a terceira sessão, tendo-se obtido níveis de ausência de dor na última sessão. A participante do estudo refere, no final das três sessões, melhorias generalizadas tendo conseguido prescindir do anti-depressivo que regularmente ingeria.peerReviewe

Status quo of pain-related patient reported outcomes and perioperative pain management in 10 415 patients from 10 countries: analysis of registry data

Postoperative pain is common at the global level, despite considerable attempts for improvement, reflecting the complexity of offering effective pain relief. In this study, clinicians from Mexico, China, and eight European countries evaluated perioperative pain practices and patient-reported outcomes (PROs) in their hospitals as a basis for carrying out quality improvement (QI) projects in each country